Cell-free protein synthesis (CFPS) transcends the limitations of living cells, unlocking unprecedented possibilities in biotechnology. This talk explores the potential of diverse applications of CFPS with constructive approach, including therapeutic protein production, screening, and manufacturing, and high-throughput platforms with AI/ML. This talk will foster an open dialogue across the CFPS space and showcase opportunities for this platform to drive synthetic biology innovation beyond the boundaries of life.

The PURE (Protein synthesis Using Recombinant Elements) system is a reconstituted cell-free protein synthesis system invented by Prof. Ueda in 2001. This session will reflect on 25 years of this groundbreaking platform, trace its evolution and explore its core principles and advantages in today's synthetic biology landscape. Learn how this critical system works synergistically with other technologies including microfluidics, high-throughput screening platforms, in vitro display, AI/ML-approach to advance applications in basic research, protein engineering, and biologics R&D. Join the original developers of this technology to explore future directions and potential novel uses of PURE system technology to revolutionize fields such as personalized medicine, biomanufacturing, and artificial cells.