The PURE (Protein synthesis Using Recombinant Elements) system is a reconstituted cell-free protein synthesis system invented by Prof. Ueda in 2001. This session will reflect on 25 years of this groundbreaking platform, trace its evolution and explore its core principles and advantages in today's synthetic biology landscape. Learn how this critical system works synergistically with other technologies including microfluidics, high-throughput screening platforms, in vitro display, AI/ML-approach to advance applications in basic research, protein engineering, and biologics R&D. Join the original developers of this technology to explore future directions and potential novel uses of PURE system technology to revolutionize fields such as personalized medicine, biomanufacturing, and artificial cells.