Description: Scala Biodesign and Ribbon Bio will present a joint workflow that helps teams move more efficiently from protein design to experimental testing in a fully cell-free workflow. In this talk, Jodi Barrientos, CEO of Ribbon Bio and Ravit Netzer, Scala Biodesign Co-founder and CEO, will share a case study involving a DNA endonuclease that was difficult to express and purify in conventional cell-based workflows, resulting in low expression and insufficient yield for further biochemical characterization.  Using ScalaOS, a focused set of engineered variants was generated to address the developability challenge. Ribbon Bio then enabled rapid conversion of those variants into high-accuracy linear DNA using MiroSynth™Cell-Free DNA without cellular constraints, helping remove a key bottleneck between design and experimental evaluation. With only 23 variants, the combined approach led to up to nine-fold higher purification yield and recovery of highly pure protein on the Nuclera eProtein Discovery instrument. More broadly, the talk will show how Scala and Ribbon complement each other in a practical customer workflow: Scala helps narrow the search to a manageable set of promising designs, while Ribbon makes it possible to build those designs quickly and reliably for downstream testing.